《植物生理学报》 2010, 46(6): 575-578

壳寡糖、一氧化氮和植物激素在烟草气孔运动中的作用及其相互关系

李艳¹, 李洪艳¹, 王青³, 李康宁¹, 张蕾蕾¹, 赵小明², 杜昱光^2,*, 侯和胜^1,*

1 辽宁师范大学生命科学学院, 辽宁大连 116029; 2 中国科学院大连化学物理研究所, 辽宁大连 116023; 3 北京市辐射中心, 北 京100875

通信作者：杜昱光；E-mail: articles1805@gmail.com, hesheng_hou@126.com；Tel: 0411-84379061;0411-84259112

摘　要：

本文研究了壳寡糖(COS)、一氧化氮(NO)和植物激素对烟草气孔运动的作用及其相互关系, 结果表明, COS、NO、 脱落酸(ABA)能诱导烟草气孔开度减小; ABA 合成抑制剂钨酸钠(Na2WO4)和 NO 合成酶抑制剂 L-NAME 具有清除 COS、 ABA 或 NO 诱导烟草气孔开度减小的作用。说明 COS 通过诱导 ABA 和 NO 产生, 进而诱导烟草气孔开度减小, 而且 ABA 和 NO 之间有相互作用。另外, 细胞分裂素和生长素能够诱导烟草气孔开度增大, 也能够逆转 COS 诱导的气孔开度减小。

关键词：壳寡糖; 一氧化氮; 脱落酸; 细胞分裂素; 生长素; 烟草

收稿：2010-01-15   修定：2010-06-01

资助：辽宁省教育厅项目(2009A427)、辽宁省自然科学基金项目(20082153)、国家高技术研究发展计划“863” 计划(2006AA10A213、2007AA091601)和中国科学院知识创新工程重要方向项目(KSCX2-YW-G-041、KSCX2-YW-N-007)。

Role and Relationship of Oligochitosan, NO and Phytohormones in Stomatal Movement of Tobacco (Nicotiana tabacum L. cv. Samsun NN)

LI Yan¹, LI Hong-Yan¹, WANG Qing³, LI Kang-Ning¹, ZHANG Lei-Lei¹, ZHAO Xiao-Ming², DU Yu-Guang^2,*, HOU He-Sheng^1,*

¹College of Life Sciences, Liaoning Normal University, Dalian, Liaoning 116029, China; ²Dalian Institute of Chemical Physics,Chinese Academy of Sciences, Dalian, Liaoning 116023, China; ³Beijing Radiation Center, Beijing 100875, China

Corresponding author: DU Yu-Guang; E-mail: articles1805@gmail.com, hesheng_hou@126.com; Tel: 0411-84379061;0411-84259112

Abstract:

Oligosaccharide, an elicitor, could induce plant resistance against biotic and abiotic stress. Oligochitosan (COS), as well as NO or ABA, could induce stomatal opening reduction in leaves of tobacco (Nicotiana tabacum cv. Samsun NN) seedlings; but Na₂WO₄, an inhibitor of ABA could scavenge the effect of reduction in stomatal opening, and so could L-NAME (a nitric oxide synthase inhibitor). The results showed that NO and ABA could be induced by COS, and there was cross-talk between NO and ABA. In addition, cytokinin and auxin could induce increase in stomatal opening of leaves of tobacco seedlings, and reverse the function of COS that induced reduction in stomatal opening of leaves of tobacco seedlings.

Key words: oligochitosan; NO; ABA; cytokinin; auxin; tobacco (Nicotiana tabacum cv. Samsun NN)